Karsten Siller, PhD

Karsten Siller, PhD

User Services, Director

Karsten’s background is in developmental cell biology, genetics and light microscopy. He leads Research Computing’s User Services team and provides technical support, training and project consultation for UVA’s centralized compute platforms. His focus areas are HPC workflows, software containers, Python programming and image processing.

Selected Publications

  • Zhang J, Wallrabe H, Siller K, Mbogo B, Cassidy T, Alam SR, Periasamy A. (2025) Measuring Metabolic Changes in Cancer Cells Using Two‐Photon Fluorescence Lifetime Imaging Microscopy and Machine‐Learning Analysis. J Biophotonics 18 (1), e202400426. doi:10.1002/jbio.202400426

  • Sun R, Siller K. (2024) HPC Container Management at the University of Virginia. Practice and Experience in Advanced Research Computing 2024, 73, 1-4. doi:10.1145/3626203.3670568

  • Zimyanin V, Magaj M, Yu CH, Gibney T, Mustafa B, Horton X , Siller K, Cueff L, Bouvrais H, Pécréaux J, Needleman D, Redemann S. (2024) Using 3D Large Scale Tomography to Study Force Generation in the Mitotic Spindle. Microscopy and Microanalysis 30 (Supplement_1), ozae044. 344. doi:10.1093/mam/ozae044.344

  • Best MN, Lim Y, Ferenc NN, Kim N, Min L, Wang DB, Sharifi K, Wasserman AE, McTavish SA, Siller KH, Jones MK, Jenkins PM, Mandell JW, Bloom GS. (2023) Extracellular Tau Oligomers Damage the Axon Initial Segment. J Alzheimers Dis. 93 (4). doi:10.3233/JAD-221284.

  • Alam SR, Wallrabe H, Christopher KG, Siller KH, Periasamy A. (2022) Characterization of mitochondrial dysfunction due to laser damage by 2-photon FLIM microscopy. Sci Rep. 12:11938. doi:10.1038/s41598-022-15639-z

  • Cao R, Wallrabe H, Siller K, Periasamy A. (2020) Optimization of FLIM imaging, fitting and analysis for auto-fluorescent NAD(P)H and FAD in cells and tissues. Methods Appl. Fluoresc. 8(2):024001. doi:10.1088/2050-6120/ab6f25.

  • Lauren K Rudenko LK, Wallrabe H, Periasamy A, Siller K, Svindrych Z, Seward ME, Best MN, Bloom GS. (2019) Intraneuronal Tau Misfolding Induced by Extracellular Amyloid-β Oligomers. J Alzheimers Dis. 71(4):1125-1138. doi:10.3233/JAD-190226.

  • Cao R, Wallrabe H, Siller K, Rehman Alam S, Periasamy A. (2019) Single-cell redox states analyzed by fluorescence lifetime metrics and tryptophan FRET interaction with NAD(P)H.
    Cytometry A. 95(1):110-121. doi:10.1002/cyto.a.23711.

  • Wallrabe H, Svindrych Z, Alam SR, Siller KH, Wang T, Kashatus D, Hu S, Periasamy A. (2018) Segmented cell analyses to measure redox states of autofluorescent NAD(P)H, FAD & Trp in cancer cells by FLIM. Sci Rep. 8(1):79. doi:10.1038/s41598-017-18634-x.

  • Janssens DH, Hamm DC, Anhezini L, Xiao Q, Siller KH, Siegrist SE, Harrison MM, Lee CY. (2017) An Hdac1/Rpd3-Poised Circuit Balances Continual Self-Renewal and Rapid Restriction of Developmental Potential during Asymmetric Stem Cell Division. Dev Cell 40(4):367-380.e7. doi:10.1016/j.devcel.2017.01.014.

  • Doyle SE, Pahl MC, Siller KH, Ardiff L, Siegrist SE. (2017) Neuroblast niche position is controlled by Phosphoinositide 3-kinase-dependent DE-Cadherin adhesion. Development 144(5):820-829. doi:10.1242/dev.136713.

  • Siller KH, Doe CQ. (2009) Spindle orientation during asymmetric cell division. Nat Cell Biol. 11(4):365-74. doi:10.1038/ncb0409-365.

  • Siller KH, Cabernard C, Doe CQ. (2006) The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts. Nat Cell Biol. 8(6):594-600. doi:10.1038/ncb1412.

  • Siller KH, Serr M, Steward R, Hays TS, Doe CQ. (2005) Live imaging of Drosophila brain neuroblasts reveals a role for Lis1/dynactin in spindle assembly and mitotic checkpoint control. Mol Biol Cell. 16(11):5127-40. doi:10.1091/mbc.e05-04-0338.

See Google Scholar for a complete list of publications.